91Ƶ

18 December 2023

Scientists, including Linda Bojmar as first author, have introduced a comprehensive protocol for the cross-platform characterization of extracellular vesicles and particles (EVPs) in both humans and mice.

The protocol aims to provide a standardized approach for analyzing EVPs, crucial for unraveling their functions and exploring biomarker potential.

Key Highlights of the Protocol

Cross-Validation Across Platforms: The protocol emphasizes the cross-validation of isolated EVPs across different platforms, ensuring robust and consistent results.

Multifaceted Analysis: Researchers can now employ various techniques for EVP analysis, including nanoparticle tracking analysis and transmission electron microscopy, allowing for the examination of EVP size, number, and morphology.

Protein Cargo Identification: The protocol introduces a novel approach to EVP protein cargo identification using liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with bioinformatic analysis.

Validation Techniques: To verify the presence of specific EVP proteins, the protocol advocates for single-particle analysis, western blot analysis, and ExoELISA, providing a comprehensive validation framework.

The significance of this protocol lies in its ability to facilitate EVP cross-validation across different platforms, enhancing the reliability of research outcomes. By offering a standardized methodology, scientists can now delve deeper into the intricate world of extracellular vesicles and particles, paving the way for breakthroughs in understanding their roles and potential as biomarkers.

This protocol is expected to catalyze advancements in the study of EVPs, opening new avenues for therapeutic development and diagnostic applications.

Contact

Latest news from LiU

LiU and Region Östergötland invest in AI and precision health

91Ƶ and Region Östergötland announce initiatives in the areas of life science, medtech, innovation and information-driven precision health in a new collaboration agreement.

A man in a lab applies water to the surface of a yellow-green material.

More effective production of “green” hydrogen with new combined material

Hydrogen produced from water is a promising renewable energy source – especially if the hydrogen is produced using sunlight. Now LiU researchers show that a combination of new materials improves the efficiency of the chemical reaction several times.

Demonstration of MR elastography.

Fatty liver – but not liver damage – common in type 2 diabetes

Six out of ten people with type 2 diabetes had fatty liver in a new study. Of these, only a small percentage had developed more severe liver disease. Type 2 diabetes in combination with obesity is linked to a greater risk.